KMID : 0811720210250050479
|
|
Korean Journal of Physiology & Pharmacology 2021 Volume.25 No. 5 p.479 ~ p.488
|
|
Docetaxel-loaded PLGA nanoparticles to increase pharmacological sensitivity in MDA-MB-231 and MCF-7 breast cancer cells
|
|
Tran Phuong
Nguyen Thu Nhan Lee Ye-Seul Tran Phan Nhan Park Jeong-Sook
|
|
Abstract
|
|
|
This study aimed to develop docetaxel (DTX) loaded poly(lactic-coglycolic acid) (PLGA) nanoparticles (DTX-NPs) and to evaluate the different pharmacological sensitivity of NPs to MCF-7 and MDA-MB-231 breast cancer cells. NPs containing DTX or coumarin-6 were prepared by the nanoprecipitation method using PLGA as a polymer and d-¥á-tocopherol polyethylene glycol 1000 succinate (TPGS) as a surfactant. The physicochemical properties of NPs were characterized. In vitro anticancer effect and cellular uptake were evaluated in breast cancer cells. The particle size and zeta potential of the DTX-NPs were 160.5 ¡¾ 3.0 nm and ?26.7 ¡¾ 0.46 mV, respectively. The encapsulation efficiency and drug loading were 81.3 ¡¾ 1.85% and 10.6 ¡¾ 0.24%, respectively. The in vitro release of DTX from the DTX-NPs was sustained at pH 7.4 containing 0.5% Tween 80. The viability of MDA-MB-231 and MCF-7 cells with DTX-NPs was 37.5 ¡¾ 0.5% and 30.3 ¡¾ 1.13%, respectively. The IC50 values of DTX-NPs were 3.92- and 6.75-fold lower than that of DTX for MDA-MB-231 cells and MCF-7 cells, respectively. The cellular uptake of coumarin-6-loaded PLGA-NPs in MCF-7 cells was significantly higher than that in MDA-MB-231 cells. The pharmacological sensitivity in breast cancer cells was higher on MCF-7 cells than on MDA-MB-231 cells. In conclusion, we successfully developed DTX-NPs that showed a great potential for the controlled release of DTX. DTX-NPs are an effective formulation for improving anticancer effect in breast cancer cells.
|
|
KEYWORD
|
|
Breast cancer, Cell viability, Docetaxel, Nanoparticles
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|